4H7D
Crystal structure of haloalkane dehalogenase LinB T81A mutant from Sphingobium sp. MI1205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Collection date | 2010-09-30 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.440, 72.057, 73.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.17837 |
| Rwork | 0.177 |
| R-free | 0.21022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h77 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 19954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | VAPOR DIFFUSION, SITTING DROP, temperature 278K |
