4H6X
Structure of Patellamide maturation protease PatG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2009-06-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.940, 66.919, 105.605 |
| Unit cell angles | 90.00, 112.17, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.166 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.080 | 0.047 | 0.443 |
| Number of reflections | 56509 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 96.5 | 99.9 | 76.9 |
| Redundancy | 7.1 | 7.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | PEG 20000, vapor diffusion, hanging drop, temperature 281K |
