4H6E
tRNA-guanine transglycosylase Y106F, C158V, V233G mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.038, 64.796, 70.530 |
| Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
| Resolution | 26.389 - 1.420 |
| R-factor | 0.1491 |
| Rwork | 0.148 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gve |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.043 | |
| Number of reflections | 72492 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 96.7 | 87.2 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Tris, 1 mM DTT, 10% DMSO, 12% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
