4H61
Structure of the Schizosaccharomyces pombe Mediator subunit Med6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9189 |
Spacegroup name | P 21 3 |
Unit cell lengths | 125.950, 125.950, 125.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.060 - 2.700 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21720 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.060 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.052 | 1.010 |
Number of reflections | 18565 | |
<I/σ(I)> | 41.4 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | 19.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 400 mM sodium citrate , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |