4H5Y
High-resolution crystal structure of Legionella pneumophila LidA (60-594)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.554, 64.468, 164.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.026 - 2.100 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.773 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.187 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 35304 | |
Completeness [%] | 99.9 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200 mM trimethylamine N-oxide, pH 8.0, 20% w/v PEG2000 MME, 1% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |