4H5L
Crystal Structure of Toscana Virus Nucleocapsid Protein Hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 |
Unit cell lengths | 50.350, 93.750, 95.520 |
Unit cell angles | 67.92, 85.94, 87.95 |
Refinement procedure
Resolution | 50.220 - 2.750 |
R-factor | 0.2199 |
Rwork | 0.219 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lyf |
RMSD bond length | 0.009 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.345 | 50.221 | 2.900 |
High resolution limit [Å] | 2.750 | 8.700 | 2.750 |
Rmerge | 0.026 | 0.474 | |
Total number of observations | 2453 | 10787 | |
Number of reflections | 39945 | ||
<I/σ(I)> | 6.8 | 22.6 | 1.4 |
Completeness [%] | 95.2 | 95.9 | 94.2 |
Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23% isopropanol, 280 mM magnesium chloride, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |