4H5B
Crystal Structure of DR_1245 from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-06-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.91983 |
Spacegroup name | P 65 |
Unit cell lengths | 91.714, 91.714, 87.746 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.900 - 2.000 |
R-factor | 0.16524 |
Rwork | 0.164 |
R-free | 0.19480 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.900 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 28448 | |
Completeness [%] | 99.8 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 1.7 M NH4(SO4)2, 27% w/v glycerol, 100 mM KBr, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |