4H4X

Crystal Structure of Ferredoxin reductase, BphA4 E175A/T176R/Q177G mutant (oxidized form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	95
Detector technology	CCD
Collection date	2012-08-04
Detector	RAYONIX MX-300
Wavelength(s)	0.97948
Spacegroup name	P 61 2 2
Unit cell lengths	98.215, 98.215, 170.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.580 - 1.500
R-factor	0.192
Rwork	0.191
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gqw
RMSD bond length	0.006
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.580	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	76902
Completeness [%]	98.3	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293	2.5M Sodium formate, 0.1M acetate buffer, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K