4H4U

Crystal Structure of Ferredoxin reductase, BphA4 T176R mutant (reduced form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-10-15
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	98.031, 98.031, 171.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.545 - 1.600
R-factor	0.2261
Rwork	0.225
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvf
RMSD bond length	0.007
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.900	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	62024
Completeness [%]	95.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293	2.5M Sodium formate, 0.1M acetate buffer, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K