4H4T

Crystal Structure of Ferredoxin reductase, BphA4 T176R mutant (oxidized form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-10-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9796
Spacegroup name	P 61 2 2
Unit cell lengths	98.332, 98.332, 170.423
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.177 - 1.500
R-factor	0.187
Rwork	0.186
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gqw
RMSD bond length	0.006
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.160	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	77482
Completeness [%]	99.0	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293	2.5M Sodium formate, 0.1M acetate buffer, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K