4H4B
Human cytosolic 5'-nucleotidase II in complex with Anthraquinone-2,6- disulfonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979696 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.110, 126.830, 130.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.920 - 2.900 |
R-factor | 0.188 |
Rwork | 0.184 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j2c |
RMSD bond length | 0.015 |
RMSD bond angle | 1.659 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 90.900 |
High resolution limit [Å] | 2.900 |
Number of reflections | 16022 |
Completeness [%] | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | magnesium sulphate and Tris buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |