4H37
Crystal structure of a voltage-gated K+ channel pore domain in a closed state in lipid membranes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.100, 80.670, 55.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.600 - 3.350 |
| R-factor | 0.318 |
| Rwork | 0.314 |
| R-free | 0.34100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eff 2atk 2A79 AND 1ORQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.116 | 0.660 |
| Number of reflections | 3736 | |
| <I/σ(I)> | 15.8 | 1.1 |
| Completeness [%] | 95.2 | 86 |
| Redundancy | 5.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 293 | 25% (v/v) PEG-MME-550, 0.35 M sodium nitrate, 50 mM ADA, 50 mM sodium malonate pH 7.0, Lipidic Cubic Phase (LCP), temperature 293K |
