4H2K
Crystal structure of the catalytic domain of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-03 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.667, 44.721, 92.481 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.840 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isz |
RMSD bond length | 0.021 |
RMSD bond angle | 2.035 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.880 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.090 | |
Number of reflections | 42453 | |
<I/σ(I)> | 10 | |
Completeness [%] | 96.5 | 97.8 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 289 | 0.2 M Ammonium acetate, 25% PEG 3350, 0.1 M BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |