4H2G

Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with adenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-29
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 2
Unit cell lengths	67.100, 131.000, 66.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.180 - 1.550
R-factor	0.12929
Rwork	0.129
R-free	0.17961
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.996
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.340
High resolution limit [Å]	1.550
Number of reflections	84660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	292	1.8 M magnesium sulfate, 0.1 M Tris-HCl , pH 8.5, VAPOR DIFFUSION, temperature 292K