4H1W
E1 structure of the (SR) Ca2+-ATPase in complex with Sarcolipin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 85.870, 139.540, 141.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.007 - 3.100 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 | |
| High resolution limit [Å] | 3.100 | 30.000 | 3.100 |
| Rmerge | 0.267 | 0.019 | 0.019 |
| Number of reflections | 31378 | 43 | 2791 |
| <I/σ(I)> | 14.19 | 49.15 | 2.21 |
| Completeness [%] | 99.3 | 84.3 | 99.2 |
| Redundancy | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 296 | PEG6000, glycerol, MgSO4, MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
