4H1U
Nucleotide-free human dynamin-1-like protein GTPase-GED fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-02 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.500, 151.430, 42.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.853 - 2.300 |
R-factor | 0.2317 |
Rwork | 0.230 |
R-free | 0.26920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3snh |
RMSD bond length | 0.005 |
RMSD bond angle | 0.976 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 75.715 | 36.715 | 2.420 |
High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
Rmerge | 0.044 | 0.449 | |
Total number of observations | 1728 | 7783 | |
Number of reflections | 15371 | ||
<I/σ(I)> | 7.9 | 11.8 | 1.7 |
Completeness [%] | 96.6 | 90.6 | 96.8 |
Redundancy | 3.5 | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M sodium citrate, 27.5% PEG 3000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |