4H1G

Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	49.463, 74.927, 98.673
Unit cell angles	90.00, 96.44, 90.00

Refinement procedure

Resolution	19.896 - 2.150
R-factor	0.1713
Rwork	0.169
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3KAR and 1MH3
RMSD bond length	0.005
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.900	19.900	2.500
High resolution limit [Å]	2.150	10.000	2.150
Rmerge	0.081	0.022	0.337
Number of reflections	38614	344	14048
<I/σ(I)>	12.02	35.38	3.83
Completeness [%]	98.8	83.1	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M Hepes, 14% PEG 4000, 75 mM NaCl, 5% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K