4H18
Three dimensional structure of corynomycoloyl tranferase C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.804, 190.688, 78.497 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.646 - 1.755 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1va5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.386 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.811 | 1.860 |
| High resolution limit [Å] | 1.755 | 1.755 |
| Rmerge | 0.154 | 0.643 |
| Number of reflections | 128495 | |
| <I/σ(I)> | 9.46 | 2.6 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.89 | 3.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 4000, 0.2M magnesium chloride, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
