4H11

Interaction partners of PSD-93 studied by X-ray crystallography and fluorescent polarization spectroscopy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.200, 45.550, 104.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.670
R-factor	0.1738
Rwork	0.172
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.253
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	19048
Completeness [%]	100.0	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	Crystallization of PSD-93 PDZ1ext was performed using the hanging-drop vapor diffusion technique by mixing 1 microliter of a 2 mg/ml protein solution (PBS buffer) and 1 l reservoir solution (0.2 M lithium sulfate, 20% PEG 1000, 0.1 M citric acid/sodium hydrogen phosphate pH 4.2. The reservoir volume was 500 microliter, VAPOR DIFFUSION, HANGING DROP, temperature 295K