4H0J
Mutant M58C of Nostoc sp Cytochrome c6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.823, 80.157, 80.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.720 - 2.000 |
| R-factor | 0.20863 |
| Rwork | 0.207 |
| R-free | 0.24586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gyd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.720 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32713 | |
| Completeness [%] | 98.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M Citrate, 2.5M Ammonium sulphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
