4H0F
Mutant Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.400, 93.990, 66.880 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hjt |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.639 |
| Number of reflections | 19430 | |
| <I/σ(I)> | 6.8 | 1.5 |
| Completeness [%] | 98.0 | 97.9 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 30% PEG2000 monomethyl ether 0.1 mM sodium citrate, 10 % ethylene glycol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
