4H0E
Crystal Structure of mutant ORR3 in complex with NTD of AraR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.800, 42.620, 67.440 |
| Unit cell angles | 90.00, 114.92, 90.00 |
Refinement procedure
| Resolution | 40.339 - 1.973 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4egz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.339 | 2.080 |
| High resolution limit [Å] | 1.973 | 1.973 |
| Number of reflections | 24656 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 298 | 25% PEG 8000, pH 4.2, VAPOR DIFFUSION, temperature 298K |
