Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 |
Unit cell lengths | 90.499, 90.499, 45.319 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.020 - 1.260 |
R-factor | 0.1284 |
Rwork | 0.127 |
R-free | 0.15340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.349 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.310 |
High resolution limit [Å] | 1.260 | 2.710 | 1.260 |
Rmerge | 0.069 | 0.056 | 0.164 |
Number of reflections | 57188 | ||
<I/σ(I)> | 16.2 | ||
Completeness [%] | 99.9 | 99.2 | 99.9 |
Redundancy | 5.6 | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2.6M AS, 50mM Tris 8.5, 1mM EDTA, vapor diffusion, hanging drop, temperature 298K |