4H09
Crystal structure of a leucine-rich repeat protein (EUBVEN_01088) from Eubacterium ventriosum ATCC 27560 at 2.50 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-03 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97925 |
Spacegroup name | P 61 |
Unit cell lengths | 105.513, 105.513, 361.567 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.965 - 2.500 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.20930 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SOLVE |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.965 | 29.965 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.049 | 0.630 | |
Total number of observations | 3278 | 22837 | |
Number of reflections | 78283 | ||
<I/σ(I)> | 9.4 | 11.1 | 1.2 |
Completeness [%] | 99.9 | 93 | 100 |
Redundancy | 4 | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M sodium citrate pH 5.6, 20% 2-propanol, 20% polyethylene glycol 4000, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |