4GZE
Crystal structure of 6-phospho-beta-glucosidase from Lactobacillus plantarum (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-24 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 31 |
Unit cell lengths | 96.086, 96.086, 289.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.310 |
R-factor | 0.17421 |
Rwork | 0.174 |
R-free | 0.21338 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qom |
RMSD bond length | 0.013 |
RMSD bond angle | 1.309 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.079 | 0.285 |
Number of reflections | 130353 | |
<I/σ(I)> | 21.8 | 2.75 |
Completeness [%] | 99.6 | 96.7 |
Redundancy | 4.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.6 M NaCl, 0.1 M MES/NaOH, 20% PEG4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |