4GZC

Crystal structure of yeast Ent2 ENTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Detector technology	CCD
Collection date	2011-06-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0333
Spacegroup name	P 21 21 21
Unit cell lengths	41.310, 53.350, 70.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.650 - 1.300
R-factor	0.21251
Rwork	0.212
R-free	0.23064
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.209
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.330
High resolution limit [Å]	1.300	1.300
Number of reflections	37547
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM Imidazole, 20% PEG 8,000, 400 mM NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K