4GYX

The von Willebrand Factor A3 domain binding region of type III collagen stabilized by the cysteine knot

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-29
Detector	NOIR-1
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	19.070, 26.990, 64.120
Unit cell angles	90.00, 92.48, 90.00

Refinement procedure

Resolution	19.050 - 1.490
R-factor	0.1805
Rwork	0.176
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dmt
RMSD bond length	0.006
RMSD bond angle	2.146
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.050	19.050	1.570
High resolution limit [Å]	1.490	4.710	1.490
Rmerge	0.083	0.068	0.383
Number of reflections	10752
<I/σ(I)>	8.2	14.5	3.2
Completeness [%]	98.9	98.4	93.1
Redundancy	3.5	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	28% PEG 3350, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K