4GYV
Crystal structure of the DH domain of FARP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979345 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.193, 209.991, 325.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.575 - 2.900 |
| R-factor | 0.1787 |
| Rwork | 0.178 |
| R-free | 0.22120 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.174 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.084 | 0.053 | 0.796 |
| Number of reflections | 91636 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 10 | 9.3 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, 1.5 M LiSO4, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
