4GYD
Nostoc sp Cytochrome c6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.721, 79.803, 80.154 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.480 - 1.800 |
R-factor | 0.18112 |
Rwork | 0.179 |
R-free | 0.21564 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.929 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CRANK (Afro/Crunch2/Bp3/DM/ARP-wARP) |
Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.480 | |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 44311 | |
Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Tris, 2.3M Ammonium sulphate, 100mM Lithium sulphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |