4GY3

T. vulcanus Phycocyanin crystallized in 2M Urea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-23
Detector	ADSC QUANTUM 315r
Spacegroup name	P 63
Unit cell lengths	153.820, 153.820, 39.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.900 - 2.500
R-factor	0.225
Rwork	0.225
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3018
RMSD bond length	0.012
RMSD bond angle	18.320
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	44.400
High resolution limit [Å]	2.500
Rmerge	0.099
Number of reflections	18972
<I/σ(I)>	13
Completeness [%]	100.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	293	0.8M ammonium sulfate, 20mM Tris pH=8 mixed 1:1 with 10mg/ml protein + 2M urea, VAPOR DIFFUSION, HANGING DROP, temperature 293K