4GY2
Crystal structure of apo-Ia-actin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.442, 135.872, 153.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.190 - 2.710 |
| R-factor | 0.20159 |
| Rwork | 0.199 |
| R-free | 0.25733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3buz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.190 | 2.750 |
| High resolution limit [Å] | 2.710 | 2.700 |
| Rmerge | 0.098 | 0.461 |
| Number of reflections | 31910 | |
| <I/σ(I)> | 9.9 | 5.1 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.13 | 18% PEG 1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K |
