4GXT
The crystal structure of a conserved functionally unknown protein from Anaerococcus prevotii DSM 20548
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.130, 53.226, 75.849 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 33.366 - 1.821 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22260 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.111 | 0.581 |
| Number of reflections | 34028 | |
| <I/σ(I)> | 25.2 | 2.8 |
| Completeness [%] | 97.4 | 95.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M Ammonium sulfate, 22% (w/v) PEG4000, 0.1M Sodium Acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
