4GXP
Chimeric Family 1 beta-glucosidase made with non-contiguous SCHEMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 115.377, 115.377, 282.538 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.430 - 3.000 |
R-factor | 0.24164 |
Rwork | 0.239 |
R-free | 0.29377 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.732 |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP ((CCP4 6.2)) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.430 | 37.429 | 3.160 |
High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
Rmerge | 0.025 | 0.381 | |
Number of reflections | 43376 | ||
<I/σ(I)> | 16.3 | 23.1 | 2 |
Completeness [%] | 99.6 | 97.5 | 99.6 |
Redundancy | 4.9 | 4.6 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 20% polyethylene glycol 3350, 0.4M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |