4GXO
Crystal structure of Staphylococcus aureus protein SarZ mutant C13E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0782 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.442, 55.792, 119.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.050 |
R-factor | 0.22438 |
Rwork | 0.222 |
R-free | 0.27020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.176 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.120 |
High resolution limit [Å] | 2.050 | 4.400 | 2.050 |
Number of reflections | 18598 | ||
Completeness [%] | 99.7 | 99.4 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 0.1 M Bis-Tris, 25% polyethylene glycol 3,350, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |