4GXF
Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.93340 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 176.660, 176.660, 176.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.010 - 2.734 |
R-factor | 0.18238 |
Rwork | 0.182 |
R-free | 0.19858 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cg8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.590 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.013 | |
High resolution limit [Å] | 2.734 | 2.730 |
Number of reflections | 59241 | |
Completeness [%] | 80.2 | 80.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 35-45% polyethylene glycol-550-monomethyl ether, 0.15-0.5M NaCl, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |