4GX9
Crystal structure of a DNA polymerase III alpha-epsilon chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.017, 56.980, 135.073 |
Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
Resolution | 35.280 - 2.150 |
R-factor | 0.22948 |
Rwork | 0.226 |
R-free | 0.29079 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hnh |
RMSD bond length | 0.019 |
RMSD bond angle | 1.745 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.095 | 0.499 |
Number of reflections | 67257 | |
<I/σ(I)> | 10.41 | 1.9 |
Completeness [%] | 97.2 | |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 279 | 0.2M MgCl2, 0.1M Tris, 16-18% PEG3350, 3mM TCEP, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 279K |