4GWX
Crystal Structure of product complexes of Porcine Liver Fructose-1,6-bisphosphatase with restrained subunit pair rotation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-10-18 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.971, 83.272, 165.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.740 - 2.350 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.26392 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cnq |
RMSD bond length | 0.021 |
RMSD bond angle | 1.838 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.440 | 41.440 | 2.430 |
High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
Rmerge | 0.094 | 0.057 | 0.313 |
Number of reflections | 15914 | ||
<I/σ(I)> | 7.8 | 14.3 | 3.2 |
Completeness [%] | 99.4 | 97.3 | 99.9 |
Redundancy | 3.53 | 3.44 | 3.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |