4GWA
Crystal Structure of a GH7 Family Cellobiohydrolase from Limnoria quadripunctata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-22 |
| Detector | PSI PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.920, 54.050, 71.970 |
| Unit cell angles | 94.13, 95.70, 94.64 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1498 |
| Rwork | 0.148 |
| R-free | 0.18410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.787 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 15.000 | 1.600 |
| Rmerge | 0.079 | 0.040 | 0.479 |
| Rmeas | 0.047 | 0.582 | |
| Number of reflections | 100073 | 108 | 16285 |
| <I/σ(I)> | 10.61 | 34.59 | 2.34 |
| Completeness [%] | 98.0 | 86.4 | 96.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M Bis-Tris, 25% PEG 3350, 0.2M MgCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
