4GW2
Crystal structure of arginine kinase in complex with L-ornithine, MgADP, and nitrate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.541 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.393, 70.312, 80.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.648 - 2.157 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.22860 |
Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1M15 |
Starting model (for MR) | 1m15 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.892 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.648 | 2.374 |
High resolution limit [Å] | 2.157 | 2.157 |
Number of reflections | 19862 | |
Completeness [%] | 96.7 | 91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Protein at 30 mg/ml, 26% PEG 6000, 0.05M HEPES, 0.1M Magnesium chloride, 0.02M Potassium ADP, 0.25M Sodium nitrate, 0.025M Sodium azide, 0.005M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 398.0K |