4GVZ

Crystal structure of arginine kinase in complex with D-arginine, MgADP, and nitrate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-13
Detector	MAR CCD 165 mm
Wavelength(s)	1.541
Spacegroup name	P 21 21 21
Unit cell lengths	64.109, 65.382, 85.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.900 - 2.960
R-factor	0.194
Rwork	0.192
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m15
RMSD bond length	0.003
RMSD bond angle	0.758
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.901
High resolution limit [Å]	2.958
Number of reflections	7468
Completeness [%]	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	Protein at 30 mg/ml, 26% PEG 6000, 0.05M HEPES, 0.1M Magnesium chloride, 0.02M Potassium ADP, 0.25M Sodium nitrate, 0.025M Sodium azide, 0.005M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K