4GVZ
Crystal structure of arginine kinase in complex with D-arginine, MgADP, and nitrate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.541 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.109, 65.382, 85.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.900 - 2.960 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m15 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.758 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.901 |
High resolution limit [Å] | 2.958 |
Number of reflections | 7468 |
Completeness [%] | 93.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Protein at 30 mg/ml, 26% PEG 6000, 0.05M HEPES, 0.1M Magnesium chloride, 0.02M Potassium ADP, 0.25M Sodium nitrate, 0.025M Sodium azide, 0.005M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |