4GVR
X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Collection date | 2011-12-08 |
Wavelength(s) | 1.0001 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.540, 122.080, 79.390 |
Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
Resolution | 48.170 - 1.520 |
R-factor | 0.16754 |
Rwork | 0.164 |
R-free | 0.23124 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qlm |
RMSD bond length | 0.018 |
RMSD bond angle | 1.890 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.170 |
High resolution limit [Å] | 1.520 |
Number of reflections | 164493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 10% (w/v) PEG 4000, 10% (v/v) Isopropanol, 0.1M Sodium acetate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |