4GVQ
X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase in complex with tetrahydromethanpterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Collection date | 2007-12-13 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.597, 94.204, 114.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.710 - 1.300 |
| R-factor | 0.13497 |
| Rwork | 0.134 |
| R-free | 0.16024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 230569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 14% (w/v) PEG 4000, 0.4M ammonium acetate, 0.1M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
