4GVP
Crystal Structure of the Response Regulator Protein VraR from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-07 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91810 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.149, 103.804, 114.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.162 - 2.030 |
R-factor | 0.1741 |
Rwork | 0.172 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.968 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.2.3) |
Refinement software | PHENIX (1.8_1066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 5.510 | 2.030 |
Rmerge | 0.096 | 0.057 | 0.607 |
Number of reflections | 60715 | ||
<I/σ(I)> | 9 | 55.5 | 3.3 |
Completeness [%] | 99.8 | 99.8 | 99.5 |
Redundancy | 7.4 | 6.7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 80 mM Tris-HCl, 160 mM ammonium acetate, 16% (w/v) PEG 4000, 6% 2-methyl-2,4-pentanediol, pH 8.5, vapor diffusion, temperature 277K |