4GVF
Crystal structure of Salmonella typhimurium family 3 glycoside hydrolase (NagZ) bound to GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-19 |
Detector | RAYONIX MX-300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.544, 66.233, 94.968 |
Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
Resolution | 48.890 - 1.350 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.066 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.890 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.060 | 0.234 |
Number of reflections | 131665 | |
<I/σ(I)> | 13.8 | 4.7 |
Completeness [%] | 99.1 | 93.7 |
Redundancy | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 296 | 0.1M MES, 25% PEG 1000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |