4GVE
Tacaribe nucleoprotein structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 4 |
| Unit cell lengths | 115.170, 115.170, 56.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.590 - 1.730 |
| R-factor | 0.15389 |
| Rwork | 0.153 |
| R-free | 0.16686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gv3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.252 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.580 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.084 | 0.780 |
| Number of reflections | 39191 | |
| <I/σ(I)> | 1.9 | 1.9 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 32% PEG3350, 0.1 M MOPS (pH7.0), and 0.11 M ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
