4GVC
Crystal Structure of T-cell Lymphoma Invasion and Metastasis-1 PDZ in complex with phosphorylated Syndecan1 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.717, 50.135, 57.712 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.850 - 1.540 |
| R-factor | 0.16717 |
| Rwork | 0.166 |
| R-free | 0.19964 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kzd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.850 | 1.620 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.030 | 0.121 |
| Number of reflections | 11440 | |
| <I/σ(I)> | 34.4 | 9.3 |
| Completeness [%] | 95.5 | 74.6 |
| Redundancy | 6.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 0.1 M sodium acetate, 25% PEG4000, 8% isopropanol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
