4GVB

Crystal structure of the virally encoded antifungal protein, KP6, heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-12
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	44.419, 44.419, 155.648
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.468 - 1.800
R-factor	0.2164
Rwork	0.215
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.965
Data reduction software	SAINT
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.1_353))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.890
High resolution limit [Å]	1.800	1.800
Number of reflections	13869
Completeness [%]	93.7	90.5
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	Reservoir solution contained 50 mM Tris, pH 8.0, 0.7 M ammonium sulfate, and 1 mM sodium azide. The drops were composed of 1:1 ratio of reservoir solution and 8.2 mg/ml KP6, VAPOR DIFFUSION, HANGING DROP, temperature 298K