4GV6
Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 176 |
Detector technology | CCD |
Collection date | 2011-06-06 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.510, 46.510, 208.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.160 - 1.980 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22900 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.001 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.540 | 2.080 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.050 | 0.175 |
Number of reflections | 16160 | |
<I/σ(I)> | 8 | 2.2 |
Completeness [%] | 89.8 | 92.4 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 18.5% PEG MME 5000, 0.1 M sodium citrate, and 2.4% PEG MME350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |