4GUO
structure of p73 DNA binding domain complex with 12 bp DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.250, 104.329, 123.324 |
| Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
| Resolution | 44.010 - 3.190 |
| R-factor | 0.21079 |
| Rwork | 0.208 |
| R-free | 0.27028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vd0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 34489 | |
| <I/σ(I)> | 19.5 | 3.1 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 0.1M MES, 0.1M NA ACETATE, 15-17% PEG 20K, pH 6.0, VAPOR DIFFUSION, temperature 298K |
