4GU7

Crystal structure of DyP-type peroxidase (SCO7193) from Streptomyces coelicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 21 3
Unit cell lengths	188.950, 188.950, 188.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.489 - 3.100
R-factor	0.1594
Rwork	0.157
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gvk
RMSD bond length	0.009
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge		0.049	0.827
Number of reflections	40721	591	5888
<I/σ(I)>	19.84	52.09	4.24
Completeness [%]	99.6	66	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Protein solution was at 15 mg/mL, containing 20 mM HEPES and 100 mM KCl. Mother liqueur contained 2.0 M (NH4)2SO4. Cryoprotectant contained 25% glycerol, pH 8.0, vapor diffusion, hanging drop, temperature 277K